Lysis:Article Title: A Processive Protein Chimera Introduces Mutations across Defined DNA Regions In Vivo
Article Snippet: Gene blocks were obtained from Integrated DNA Technologies. .. Reagents: The following reagents were obtained as indicated: Kanamycin monosulfate, fosfomycin, agar, and chloramphenicol (Alfa Aesar J61272, J66602, A10752, and B20841, respectively); tetracycline hydrochloride (CalBioChem 58346); rifampicin (TCI R0079); ampicillin (Fisher Bioreagents BP1760-25); streptomycin sulfate (MP Biomedical 100556); tetrazolium chloride, L-rhamnose, antifoam-204, and ethyl methanesulfonate (Sigma-Aldrich T8877, W373011, A8311, and M0880, respectively); L-arabinose and cycloheximide (Chem-Impex 01654 and 00083, respectively); and lysogeny broth (LB; Difco 244620); anhydrous sodium phosphate dibasic and monobasic sodium phosphate (Mallinckrodt 7917 and 7892, respectively); potassium chloride and isopropyl β-D-1-thiogalactopyranoside (Sigma P9333 and I6758, respectively); magnesium sulfate (Macron 6070-12); o-Nitrophenyl-β-galactoside and egg-white lysozyme (VWR 0789 and 0663, respectively); PopCulture lysis reagent (EMD Millipore 71092- 4); 2-mercaptoethanol (Bio-Rad 161-0710); trimethoprim (Matrix Scientific 058373). ..
Diffusion-based Assay:Article Title: Characterization of urinary Escherichia coli isolates in HIV-seropositive women with asymptomatic bacteriuria in Western Ghana.
Article Snippet: The final 126 identities of isolates were confirmed via MALDI-TOF Biotyper (Bruker 127 Daltonics, Bremen, Germany) at the Department of Medical Microbiology 128 of the University of Ghana Medical School (UGMS). .. 136 Antimicrobial susceptibility testing was performed using the Kirby-Bauer 137 disc diffusion method with the following antibiotics discs; trimethoprim- 138 sulfamethoxazole (cotrimoxazole) (25 μg), nitrofurantoin (300 μg), 139 nalidixic acid (30 μg), cefuroxime (30 μg), ampicillin (10 μg), fosfomycin 140 (200 μg), tetracycline (30 μg), ciprofloxacin (5 μg), gentamicin (10 μg), 141 cefepime (30 μg), amikacin (30 μg), meropenem (30 μg), ceftriaxone (30 142 μg), and amoxiclav (30 μg) (Oxoid Ltd. Basingstoke, UK). .. Calibration was done 133 using the Bruker Bacterial Test Standard according to the 134 manufacturer’s instructions.
Article Title: IncL plasmid-mediated dissemination of OXA-48 β-lactamase and bla CTX-M-15 gene amplification identified via long-read sequencing in carbapenem-resistant Enterobacterales
Article Snippet: Clinical and Laboratory Standards Institute (CLSI) breakpoints were applied for cefoperazone, netilmicin and tetracycline., Additionally, in carbapenemase-producing Enterobacterales (CPE) isolates, susceptibility to novel β-lactam-β-lactamase inhibitor combinations (βL-βLIs), including meropenem–vaborbactam, imipenem–relebactam, ceftazidime–avibactam and ceftolozane–tazobactam, was determined using the MIC test strip (bioMérieux, France). .. Susceptibility to cefiderocol was tested by the disc diffusion (Oxoid, UK) and to fosfomycin using the MIC test strip (Liofilchem, Italy). ..
Stripping Membranes:Article Title: IncL plasmid-mediated dissemination of OXA-48 β-lactamase and bla CTX-M-15 gene amplification identified via long-read sequencing in carbapenem-resistant Enterobacterales
Article Snippet: Clinical and Laboratory Standards Institute (CLSI) breakpoints were applied for cefoperazone, netilmicin and tetracycline., Additionally, in carbapenemase-producing Enterobacterales (CPE) isolates, susceptibility to novel β-lactam-β-lactamase inhibitor combinations (βL-βLIs), including meropenem–vaborbactam, imipenem–relebactam, ceftazidime–avibactam and ceftolozane–tazobactam, was determined using the MIC test strip (bioMérieux, France). .. Susceptibility to cefiderocol was tested by the disc diffusion (Oxoid, UK) and to fosfomycin using the MIC test strip (Liofilchem, Italy). ..
other:
Polymerase Chain Reaction:Article Title: An investigation of gene dosage reveals that increased sensitivity to D-cycloserine divergently impacts the transience of heteroresistance in Escherichia coli
Article Snippet: .. Product/component Manufacturer Difco LB Broth, Miller (Luria-Bertani) (Premix) Thermo Fisher BBL Cation-Adjust Mueller Hinton II Agar Premix Thermo Fisher Tryptone Fisher Bioreagents Yeast Extract Fisher Bioreagents Agar (80-100 mesh) Fisher Bioreagents D-glucose Fisher Chemical L-arabinose Alfa Aesar Glycerol Sigma Aldrich Chlorform Fisher Chemical Sodium Citrate Dihydrate (Na3C6H5O7*2H2O) Fisher Scientific Potassium Chloride (KCl) Fisher Sodium Chloride (NaCl) Fisher Bioreagents Calcium Chloride Dihydrate (CaCl2) Fisher Bioreagents Magnesium Sulfate Heptahydrate (MgSO4*7H2O) MP Biochemicals Sodium Phosphate Dibasic Heptahydrate (Na2HPO4*7H2O) MP Biochemicals Potassium dihyrogen Phosphate (KH2PO4) Alfa Aesar Ammonium Chloride (NH4Cl) MP biochemicals Gluose-6-phosphate (G6P) Sigma Nicotinamide (NIC) Sigma Aldrich Ciprofloxacin (CIP) Sigma Fosfomycin disodium salt (FOS) Sigma Aldrich Tobramycin sulfate (TOB) Sigma Aldrich D-cycloserine (DCS) Sigma Aldrich Anhydrotetracycline hydrochloride (aTET) Sigma Aldrich Kanamycin sulfate (KAN) Gibco Ampicillin (AMP) Acros Organics Lysozyme, chicken egg white Thermo Fisher Tris Hydrochloride (1 M, pH 8.0, DNase, RNase, and Protease Free) Fisher Bioreagents Ethylenediamine Tetraacetate Acid (EDTA) (for DNA work) (0.5 M, pH 8.0, RNase and Protease Free) Fisher Bioreagents Water (nuclease-free, 0.1 μm filtered, for molecular biology) Sigma Aldrich Gibson Assembly Master Mix New Englad Biolabs Phusion High Fidelity PCR Kit New Englad Biolabs Taq DNA Polymerase New Englad Biolabs EcoRI-HF Restriction Enzyme New Englad Biolabs 10x rCutSmart Buffer New Englad Biolabs Q5 Site-Directed Mutagenesis Kit New Englad Biolabs HiScibe T7 RNA Synthesis Kit New Englad Biolabs TaqMan Reverse Transcription Reagents Thermo Fisher Scientific Power SYBR Green qPCR Master Mix Applied Biosystems Qiaprep Spin Miniprep Kit Qiagen Qiaquick Gel Extraction Kit Qiagen Dneasy Blood & Tissue Kit Qiagen RNase-free DNase Kit Qiagen RNeasy Mini Kit Qiagen RNAprotect Bacteria Reagent Qiagen Plasmid Relevant description† PCR/Sequence Check(s)* Source pUA66 Contains promoterless gfp with rrnB T1 terminator, kanR resistance cassette, and pSC101 ori. .. Product/component Manufacturer Difco LB Broth, Miller (Luria-Bertani) (Premix) Thermo Fisher BBL Cation-Adjust Mueller Hinton II Agar Premix Thermo Fisher Tryptone Fisher Bioreagents Yeast Extract Fisher Bioreagents Agar (80-100 mesh) Fisher Bioreagents D-glucose Fisher Chemical L-arabinose Alfa Aesar Glycerol Sigma Aldrich Chlorform Fisher Chemical Sodium Citrate Dihydrate (Na3C6H5O7*2H2O) Fisher Scientific Potassium Chloride (KCl) Fisher Sodium Chloride (NaCl) Fisher Bioreagents Calcium Chloride Dihydrate (CaCl2) Fisher Bioreagents Magnesium Sulfate Heptahydrate (MgSO4*7H2O) MP Biochemicals Sodium Phosphate Dibasic Heptahydrate (Na2HPO4*7H2O) MP Biochemicals Potassium dihyrogen Phosphate (KH2PO4) Alfa Aesar Ammonium Chloride (NH4Cl) MP biochemicals Gluose-6-phosphate (G6P) Sigma Nicotinamide (NIC) Sigma Aldrich Ciprofloxacin (CIP) Sigma Fosfomycin disodium salt (FOS) Sigma Aldrich Tobramycin sulfate (TOB) Sigma Aldrich D-cycloserine (DCS) Sigma Aldrich Anhydrotetracycline hydrochloride (aTET) Sigma Aldrich Kanamycin sulfate (KAN) Gibco Ampicillin (AMP) Acros Organics Lysozyme, chicken egg white Thermo Fisher Tris Hydrochloride (1 M, pH 8.0, DNase, RNase, and Protease Free) Fisher Bioreagents Ethylenediamine Tetraacetate Acid (EDTA) (for DNA work) (0.5 M, pH 8.0, RNase and Protease Free) Fisher Bioreagents Water (nuclease-free, 0.1 μm filtered, for molecular biology) Sigma Aldrich Gibson Assembly Master Mix New Englad Biolabs Phusion High Fidelity PCR Kit New Englad Biolabs Taq DNA Polymerase New Englad Biolabs EcoRI-HF Restriction Enzyme New Englad Biolabs 10x rCutSmart Buffer New Englad Biolabs Q5 Site-Directed Mutagenesis Kit New Englad Biolabs HiScibe T7 RNA Synthesis Kit New Englad Biolabs TaqMan Reverse Transcription Reagents Thermo Fisher Scientific Power SYBR Green qPCR Master Mix Applied Biosystems Qiaprep Spin Miniprep Kit Qiagen Qiaquick Gel Extraction Kit Qiagen Dneasy Blood & Tissue Kit Qiagen RNase-free DNase Kit Qiagen RNeasy Mini Kit Qiagen RNAprotect Bacteria Reagent Qiagen Plasmid Relevant description† PCR/Sequence Check(s)* Source pUA66 Contains promoterless gfp with rrnB T1 terminator, kanR resistance cassette, and pSC101 ori.
Mutagenesis:Article Title: An investigation of gene dosage reveals that increased sensitivity to D-cycloserine divergently impacts the transience of heteroresistance in Escherichia coli
Article Snippet: .. Product/component Manufacturer Difco LB Broth, Miller (Luria-Bertani) (Premix) Thermo Fisher BBL Cation-Adjust Mueller Hinton II Agar Premix Thermo Fisher Tryptone Fisher Bioreagents Yeast Extract Fisher Bioreagents Agar (80-100 mesh) Fisher Bioreagents D-glucose Fisher Chemical L-arabinose Alfa Aesar Glycerol Sigma Aldrich Chlorform Fisher Chemical Sodium Citrate Dihydrate (Na3C6H5O7*2H2O) Fisher Scientific Potassium Chloride (KCl) Fisher Sodium Chloride (NaCl) Fisher Bioreagents Calcium Chloride Dihydrate (CaCl2) Fisher Bioreagents Magnesium Sulfate Heptahydrate (MgSO4*7H2O) MP Biochemicals Sodium Phosphate Dibasic Heptahydrate (Na2HPO4*7H2O) MP Biochemicals Potassium dihyrogen Phosphate (KH2PO4) Alfa Aesar Ammonium Chloride (NH4Cl) MP biochemicals Gluose-6-phosphate (G6P) Sigma Nicotinamide (NIC) Sigma Aldrich Ciprofloxacin (CIP) Sigma Fosfomycin disodium salt (FOS) Sigma Aldrich Tobramycin sulfate (TOB) Sigma Aldrich D-cycloserine (DCS) Sigma Aldrich Anhydrotetracycline hydrochloride (aTET) Sigma Aldrich Kanamycin sulfate (KAN) Gibco Ampicillin (AMP) Acros Organics Lysozyme, chicken egg white Thermo Fisher Tris Hydrochloride (1 M, pH 8.0, DNase, RNase, and Protease Free) Fisher Bioreagents Ethylenediamine Tetraacetate Acid (EDTA) (for DNA work) (0.5 M, pH 8.0, RNase and Protease Free) Fisher Bioreagents Water (nuclease-free, 0.1 μm filtered, for molecular biology) Sigma Aldrich Gibson Assembly Master Mix New Englad Biolabs Phusion High Fidelity PCR Kit New Englad Biolabs Taq DNA Polymerase New Englad Biolabs EcoRI-HF Restriction Enzyme New Englad Biolabs 10x rCutSmart Buffer New Englad Biolabs Q5 Site-Directed Mutagenesis Kit New Englad Biolabs HiScibe T7 RNA Synthesis Kit New Englad Biolabs TaqMan Reverse Transcription Reagents Thermo Fisher Scientific Power SYBR Green qPCR Master Mix Applied Biosystems Qiaprep Spin Miniprep Kit Qiagen Qiaquick Gel Extraction Kit Qiagen Dneasy Blood & Tissue Kit Qiagen RNase-free DNase Kit Qiagen RNeasy Mini Kit Qiagen RNAprotect Bacteria Reagent Qiagen Plasmid Relevant description† PCR/Sequence Check(s)* Source pUA66 Contains promoterless gfp with rrnB T1 terminator, kanR resistance cassette, and pSC101 ori. .. Product/component Manufacturer Difco LB Broth, Miller (Luria-Bertani) (Premix) Thermo Fisher BBL Cation-Adjust Mueller Hinton II Agar Premix Thermo Fisher Tryptone Fisher Bioreagents Yeast Extract Fisher Bioreagents Agar (80-100 mesh) Fisher Bioreagents D-glucose Fisher Chemical L-arabinose Alfa Aesar Glycerol Sigma Aldrich Chlorform Fisher Chemical Sodium Citrate Dihydrate (Na3C6H5O7*2H2O) Fisher Scientific Potassium Chloride (KCl) Fisher Sodium Chloride (NaCl) Fisher Bioreagents Calcium Chloride Dihydrate (CaCl2) Fisher Bioreagents Magnesium Sulfate Heptahydrate (MgSO4*7H2O) MP Biochemicals Sodium Phosphate Dibasic Heptahydrate (Na2HPO4*7H2O) MP Biochemicals Potassium dihyrogen Phosphate (KH2PO4) Alfa Aesar Ammonium Chloride (NH4Cl) MP biochemicals Gluose-6-phosphate (G6P) Sigma Nicotinamide (NIC) Sigma Aldrich Ciprofloxacin (CIP) Sigma Fosfomycin disodium salt (FOS) Sigma Aldrich Tobramycin sulfate (TOB) Sigma Aldrich D-cycloserine (DCS) Sigma Aldrich Anhydrotetracycline hydrochloride (aTET) Sigma Aldrich Kanamycin sulfate (KAN) Gibco Ampicillin (AMP) Acros Organics Lysozyme, chicken egg white Thermo Fisher Tris Hydrochloride (1 M, pH 8.0, DNase, RNase, and Protease Free) Fisher Bioreagents Ethylenediamine Tetraacetate Acid (EDTA) (for DNA work) (0.5 M, pH 8.0, RNase and Protease Free) Fisher Bioreagents Water (nuclease-free, 0.1 μm filtered, for molecular biology) Sigma Aldrich Gibson Assembly Master Mix New Englad Biolabs Phusion High Fidelity PCR Kit New Englad Biolabs Taq DNA Polymerase New Englad Biolabs EcoRI-HF Restriction Enzyme New Englad Biolabs 10x rCutSmart Buffer New Englad Biolabs Q5 Site-Directed Mutagenesis Kit New Englad Biolabs HiScibe T7 RNA Synthesis Kit New Englad Biolabs TaqMan Reverse Transcription Reagents Thermo Fisher Scientific Power SYBR Green qPCR Master Mix Applied Biosystems Qiaprep Spin Miniprep Kit Qiagen Qiaquick Gel Extraction Kit Qiagen Dneasy Blood & Tissue Kit Qiagen RNase-free DNase Kit Qiagen RNeasy Mini Kit Qiagen RNAprotect Bacteria Reagent Qiagen Plasmid Relevant description† PCR/Sequence Check(s)* Source pUA66 Contains promoterless gfp with rrnB T1 terminator, kanR resistance cassette, and pSC101 ori.
Reverse Transcription:Article Title: An investigation of gene dosage reveals that increased sensitivity to D-cycloserine divergently impacts the transience of heteroresistance in Escherichia coli
Article Snippet: .. Product/component Manufacturer Difco LB Broth, Miller (Luria-Bertani) (Premix) Thermo Fisher BBL Cation-Adjust Mueller Hinton II Agar Premix Thermo Fisher Tryptone Fisher Bioreagents Yeast Extract Fisher Bioreagents Agar (80-100 mesh) Fisher Bioreagents D-glucose Fisher Chemical L-arabinose Alfa Aesar Glycerol Sigma Aldrich Chlorform Fisher Chemical Sodium Citrate Dihydrate (Na3C6H5O7*2H2O) Fisher Scientific Potassium Chloride (KCl) Fisher Sodium Chloride (NaCl) Fisher Bioreagents Calcium Chloride Dihydrate (CaCl2) Fisher Bioreagents Magnesium Sulfate Heptahydrate (MgSO4*7H2O) MP Biochemicals Sodium Phosphate Dibasic Heptahydrate (Na2HPO4*7H2O) MP Biochemicals Potassium dihyrogen Phosphate (KH2PO4) Alfa Aesar Ammonium Chloride (NH4Cl) MP biochemicals Gluose-6-phosphate (G6P) Sigma Nicotinamide (NIC) Sigma Aldrich Ciprofloxacin (CIP) Sigma Fosfomycin disodium salt (FOS) Sigma Aldrich Tobramycin sulfate (TOB) Sigma Aldrich D-cycloserine (DCS) Sigma Aldrich Anhydrotetracycline hydrochloride (aTET) Sigma Aldrich Kanamycin sulfate (KAN) Gibco Ampicillin (AMP) Acros Organics Lysozyme, chicken egg white Thermo Fisher Tris Hydrochloride (1 M, pH 8.0, DNase, RNase, and Protease Free) Fisher Bioreagents Ethylenediamine Tetraacetate Acid (EDTA) (for DNA work) (0.5 M, pH 8.0, RNase and Protease Free) Fisher Bioreagents Water (nuclease-free, 0.1 μm filtered, for molecular biology) Sigma Aldrich Gibson Assembly Master Mix New Englad Biolabs Phusion High Fidelity PCR Kit New Englad Biolabs Taq DNA Polymerase New Englad Biolabs EcoRI-HF Restriction Enzyme New Englad Biolabs 10x rCutSmart Buffer New Englad Biolabs Q5 Site-Directed Mutagenesis Kit New Englad Biolabs HiScibe T7 RNA Synthesis Kit New Englad Biolabs TaqMan Reverse Transcription Reagents Thermo Fisher Scientific Power SYBR Green qPCR Master Mix Applied Biosystems Qiaprep Spin Miniprep Kit Qiagen Qiaquick Gel Extraction Kit Qiagen Dneasy Blood & Tissue Kit Qiagen RNase-free DNase Kit Qiagen RNeasy Mini Kit Qiagen RNAprotect Bacteria Reagent Qiagen Plasmid Relevant description† PCR/Sequence Check(s)* Source pUA66 Contains promoterless gfp with rrnB T1 terminator, kanR resistance cassette, and pSC101 ori. .. Product/component Manufacturer Difco LB Broth, Miller (Luria-Bertani) (Premix) Thermo Fisher BBL Cation-Adjust Mueller Hinton II Agar Premix Thermo Fisher Tryptone Fisher Bioreagents Yeast Extract Fisher Bioreagents Agar (80-100 mesh) Fisher Bioreagents D-glucose Fisher Chemical L-arabinose Alfa Aesar Glycerol Sigma Aldrich Chlorform Fisher Chemical Sodium Citrate Dihydrate (Na3C6H5O7*2H2O) Fisher Scientific Potassium Chloride (KCl) Fisher Sodium Chloride (NaCl) Fisher Bioreagents Calcium Chloride Dihydrate (CaCl2) Fisher Bioreagents Magnesium Sulfate Heptahydrate (MgSO4*7H2O) MP Biochemicals Sodium Phosphate Dibasic Heptahydrate (Na2HPO4*7H2O) MP Biochemicals Potassium dihyrogen Phosphate (KH2PO4) Alfa Aesar Ammonium Chloride (NH4Cl) MP biochemicals Gluose-6-phosphate (G6P) Sigma Nicotinamide (NIC) Sigma Aldrich Ciprofloxacin (CIP) Sigma Fosfomycin disodium salt (FOS) Sigma Aldrich Tobramycin sulfate (TOB) Sigma Aldrich D-cycloserine (DCS) Sigma Aldrich Anhydrotetracycline hydrochloride (aTET) Sigma Aldrich Kanamycin sulfate (KAN) Gibco Ampicillin (AMP) Acros Organics Lysozyme, chicken egg white Thermo Fisher Tris Hydrochloride (1 M, pH 8.0, DNase, RNase, and Protease Free) Fisher Bioreagents Ethylenediamine Tetraacetate Acid (EDTA) (for DNA work) (0.5 M, pH 8.0, RNase and Protease Free) Fisher Bioreagents Water (nuclease-free, 0.1 μm filtered, for molecular biology) Sigma Aldrich Gibson Assembly Master Mix New Englad Biolabs Phusion High Fidelity PCR Kit New Englad Biolabs Taq DNA Polymerase New Englad Biolabs EcoRI-HF Restriction Enzyme New Englad Biolabs 10x rCutSmart Buffer New Englad Biolabs Q5 Site-Directed Mutagenesis Kit New Englad Biolabs HiScibe T7 RNA Synthesis Kit New Englad Biolabs TaqMan Reverse Transcription Reagents Thermo Fisher Scientific Power SYBR Green qPCR Master Mix Applied Biosystems Qiaprep Spin Miniprep Kit Qiagen Qiaquick Gel Extraction Kit Qiagen Dneasy Blood & Tissue Kit Qiagen RNase-free DNase Kit Qiagen RNeasy Mini Kit Qiagen RNAprotect Bacteria Reagent Qiagen Plasmid Relevant description† PCR/Sequence Check(s)* Source pUA66 Contains promoterless gfp with rrnB T1 terminator, kanR resistance cassette, and pSC101 ori.
SYBR Green Assay:Article Title: An investigation of gene dosage reveals that increased sensitivity to D-cycloserine divergently impacts the transience of heteroresistance in Escherichia coli
Article Snippet: .. Product/component Manufacturer Difco LB Broth, Miller (Luria-Bertani) (Premix) Thermo Fisher BBL Cation-Adjust Mueller Hinton II Agar Premix Thermo Fisher Tryptone Fisher Bioreagents Yeast Extract Fisher Bioreagents Agar (80-100 mesh) Fisher Bioreagents D-glucose Fisher Chemical L-arabinose Alfa Aesar Glycerol Sigma Aldrich Chlorform Fisher Chemical Sodium Citrate Dihydrate (Na3C6H5O7*2H2O) Fisher Scientific Potassium Chloride (KCl) Fisher Sodium Chloride (NaCl) Fisher Bioreagents Calcium Chloride Dihydrate (CaCl2) Fisher Bioreagents Magnesium Sulfate Heptahydrate (MgSO4*7H2O) MP Biochemicals Sodium Phosphate Dibasic Heptahydrate (Na2HPO4*7H2O) MP Biochemicals Potassium dihyrogen Phosphate (KH2PO4) Alfa Aesar Ammonium Chloride (NH4Cl) MP biochemicals Gluose-6-phosphate (G6P) Sigma Nicotinamide (NIC) Sigma Aldrich Ciprofloxacin (CIP) Sigma Fosfomycin disodium salt (FOS) Sigma Aldrich Tobramycin sulfate (TOB) Sigma Aldrich D-cycloserine (DCS) Sigma Aldrich Anhydrotetracycline hydrochloride (aTET) Sigma Aldrich Kanamycin sulfate (KAN) Gibco Ampicillin (AMP) Acros Organics Lysozyme, chicken egg white Thermo Fisher Tris Hydrochloride (1 M, pH 8.0, DNase, RNase, and Protease Free) Fisher Bioreagents Ethylenediamine Tetraacetate Acid (EDTA) (for DNA work) (0.5 M, pH 8.0, RNase and Protease Free) Fisher Bioreagents Water (nuclease-free, 0.1 μm filtered, for molecular biology) Sigma Aldrich Gibson Assembly Master Mix New Englad Biolabs Phusion High Fidelity PCR Kit New Englad Biolabs Taq DNA Polymerase New Englad Biolabs EcoRI-HF Restriction Enzyme New Englad Biolabs 10x rCutSmart Buffer New Englad Biolabs Q5 Site-Directed Mutagenesis Kit New Englad Biolabs HiScibe T7 RNA Synthesis Kit New Englad Biolabs TaqMan Reverse Transcription Reagents Thermo Fisher Scientific Power SYBR Green qPCR Master Mix Applied Biosystems Qiaprep Spin Miniprep Kit Qiagen Qiaquick Gel Extraction Kit Qiagen Dneasy Blood & Tissue Kit Qiagen RNase-free DNase Kit Qiagen RNeasy Mini Kit Qiagen RNAprotect Bacteria Reagent Qiagen Plasmid Relevant description† PCR/Sequence Check(s)* Source pUA66 Contains promoterless gfp with rrnB T1 terminator, kanR resistance cassette, and pSC101 ori. .. Product/component Manufacturer Difco LB Broth, Miller (Luria-Bertani) (Premix) Thermo Fisher BBL Cation-Adjust Mueller Hinton II Agar Premix Thermo Fisher Tryptone Fisher Bioreagents Yeast Extract Fisher Bioreagents Agar (80-100 mesh) Fisher Bioreagents D-glucose Fisher Chemical L-arabinose Alfa Aesar Glycerol Sigma Aldrich Chlorform Fisher Chemical Sodium Citrate Dihydrate (Na3C6H5O7*2H2O) Fisher Scientific Potassium Chloride (KCl) Fisher Sodium Chloride (NaCl) Fisher Bioreagents Calcium Chloride Dihydrate (CaCl2) Fisher Bioreagents Magnesium Sulfate Heptahydrate (MgSO4*7H2O) MP Biochemicals Sodium Phosphate Dibasic Heptahydrate (Na2HPO4*7H2O) MP Biochemicals Potassium dihyrogen Phosphate (KH2PO4) Alfa Aesar Ammonium Chloride (NH4Cl) MP biochemicals Gluose-6-phosphate (G6P) Sigma Nicotinamide (NIC) Sigma Aldrich Ciprofloxacin (CIP) Sigma Fosfomycin disodium salt (FOS) Sigma Aldrich Tobramycin sulfate (TOB) Sigma Aldrich D-cycloserine (DCS) Sigma Aldrich Anhydrotetracycline hydrochloride (aTET) Sigma Aldrich Kanamycin sulfate (KAN) Gibco Ampicillin (AMP) Acros Organics Lysozyme, chicken egg white Thermo Fisher Tris Hydrochloride (1 M, pH 8.0, DNase, RNase, and Protease Free) Fisher Bioreagents Ethylenediamine Tetraacetate Acid (EDTA) (for DNA work) (0.5 M, pH 8.0, RNase and Protease Free) Fisher Bioreagents Water (nuclease-free, 0.1 μm filtered, for molecular biology) Sigma Aldrich Gibson Assembly Master Mix New Englad Biolabs Phusion High Fidelity PCR Kit New Englad Biolabs Taq DNA Polymerase New Englad Biolabs EcoRI-HF Restriction Enzyme New Englad Biolabs 10x rCutSmart Buffer New Englad Biolabs Q5 Site-Directed Mutagenesis Kit New Englad Biolabs HiScibe T7 RNA Synthesis Kit New Englad Biolabs TaqMan Reverse Transcription Reagents Thermo Fisher Scientific Power SYBR Green qPCR Master Mix Applied Biosystems Qiaprep Spin Miniprep Kit Qiagen Qiaquick Gel Extraction Kit Qiagen Dneasy Blood & Tissue Kit Qiagen RNase-free DNase Kit Qiagen RNeasy Mini Kit Qiagen RNAprotect Bacteria Reagent Qiagen Plasmid Relevant description† PCR/Sequence Check(s)* Source pUA66 Contains promoterless gfp with rrnB T1 terminator, kanR resistance cassette, and pSC101 ori.
Real-time Polymerase Chain Reaction:Article Title: An investigation of gene dosage reveals that increased sensitivity to D-cycloserine divergently impacts the transience of heteroresistance in Escherichia coli
Article Snippet: .. Product/component Manufacturer Difco LB Broth, Miller (Luria-Bertani) (Premix) Thermo Fisher BBL Cation-Adjust Mueller Hinton II Agar Premix Thermo Fisher Tryptone Fisher Bioreagents Yeast Extract Fisher Bioreagents Agar (80-100 mesh) Fisher Bioreagents D-glucose Fisher Chemical L-arabinose Alfa Aesar Glycerol Sigma Aldrich Chlorform Fisher Chemical Sodium Citrate Dihydrate (Na3C6H5O7*2H2O) Fisher Scientific Potassium Chloride (KCl) Fisher Sodium Chloride (NaCl) Fisher Bioreagents Calcium Chloride Dihydrate (CaCl2) Fisher Bioreagents Magnesium Sulfate Heptahydrate (MgSO4*7H2O) MP Biochemicals Sodium Phosphate Dibasic Heptahydrate (Na2HPO4*7H2O) MP Biochemicals Potassium dihyrogen Phosphate (KH2PO4) Alfa Aesar Ammonium Chloride (NH4Cl) MP biochemicals Gluose-6-phosphate (G6P) Sigma Nicotinamide (NIC) Sigma Aldrich Ciprofloxacin (CIP) Sigma Fosfomycin disodium salt (FOS) Sigma Aldrich Tobramycin sulfate (TOB) Sigma Aldrich D-cycloserine (DCS) Sigma Aldrich Anhydrotetracycline hydrochloride (aTET) Sigma Aldrich Kanamycin sulfate (KAN) Gibco Ampicillin (AMP) Acros Organics Lysozyme, chicken egg white Thermo Fisher Tris Hydrochloride (1 M, pH 8.0, DNase, RNase, and Protease Free) Fisher Bioreagents Ethylenediamine Tetraacetate Acid (EDTA) (for DNA work) (0.5 M, pH 8.0, RNase and Protease Free) Fisher Bioreagents Water (nuclease-free, 0.1 μm filtered, for molecular biology) Sigma Aldrich Gibson Assembly Master Mix New Englad Biolabs Phusion High Fidelity PCR Kit New Englad Biolabs Taq DNA Polymerase New Englad Biolabs EcoRI-HF Restriction Enzyme New Englad Biolabs 10x rCutSmart Buffer New Englad Biolabs Q5 Site-Directed Mutagenesis Kit New Englad Biolabs HiScibe T7 RNA Synthesis Kit New Englad Biolabs TaqMan Reverse Transcription Reagents Thermo Fisher Scientific Power SYBR Green qPCR Master Mix Applied Biosystems Qiaprep Spin Miniprep Kit Qiagen Qiaquick Gel Extraction Kit Qiagen Dneasy Blood & Tissue Kit Qiagen RNase-free DNase Kit Qiagen RNeasy Mini Kit Qiagen RNAprotect Bacteria Reagent Qiagen Plasmid Relevant description† PCR/Sequence Check(s)* Source pUA66 Contains promoterless gfp with rrnB T1 terminator, kanR resistance cassette, and pSC101 ori. .. Product/component Manufacturer Difco LB Broth, Miller (Luria-Bertani) (Premix) Thermo Fisher BBL Cation-Adjust Mueller Hinton II Agar Premix Thermo Fisher Tryptone Fisher Bioreagents Yeast Extract Fisher Bioreagents Agar (80-100 mesh) Fisher Bioreagents D-glucose Fisher Chemical L-arabinose Alfa Aesar Glycerol Sigma Aldrich Chlorform Fisher Chemical Sodium Citrate Dihydrate (Na3C6H5O7*2H2O) Fisher Scientific Potassium Chloride (KCl) Fisher Sodium Chloride (NaCl) Fisher Bioreagents Calcium Chloride Dihydrate (CaCl2) Fisher Bioreagents Magnesium Sulfate Heptahydrate (MgSO4*7H2O) MP Biochemicals Sodium Phosphate Dibasic Heptahydrate (Na2HPO4*7H2O) MP Biochemicals Potassium dihyrogen Phosphate (KH2PO4) Alfa Aesar Ammonium Chloride (NH4Cl) MP biochemicals Gluose-6-phosphate (G6P) Sigma Nicotinamide (NIC) Sigma Aldrich Ciprofloxacin (CIP) Sigma Fosfomycin disodium salt (FOS) Sigma Aldrich Tobramycin sulfate (TOB) Sigma Aldrich D-cycloserine (DCS) Sigma Aldrich Anhydrotetracycline hydrochloride (aTET) Sigma Aldrich Kanamycin sulfate (KAN) Gibco Ampicillin (AMP) Acros Organics Lysozyme, chicken egg white Thermo Fisher Tris Hydrochloride (1 M, pH 8.0, DNase, RNase, and Protease Free) Fisher Bioreagents Ethylenediamine Tetraacetate Acid (EDTA) (for DNA work) (0.5 M, pH 8.0, RNase and Protease Free) Fisher Bioreagents Water (nuclease-free, 0.1 μm filtered, for molecular biology) Sigma Aldrich Gibson Assembly Master Mix New Englad Biolabs Phusion High Fidelity PCR Kit New Englad Biolabs Taq DNA Polymerase New Englad Biolabs EcoRI-HF Restriction Enzyme New Englad Biolabs 10x rCutSmart Buffer New Englad Biolabs Q5 Site-Directed Mutagenesis Kit New Englad Biolabs HiScibe T7 RNA Synthesis Kit New Englad Biolabs TaqMan Reverse Transcription Reagents Thermo Fisher Scientific Power SYBR Green qPCR Master Mix Applied Biosystems Qiaprep Spin Miniprep Kit Qiagen Qiaquick Gel Extraction Kit Qiagen Dneasy Blood & Tissue Kit Qiagen RNase-free DNase Kit Qiagen RNeasy Mini Kit Qiagen RNAprotect Bacteria Reagent Qiagen Plasmid Relevant description† PCR/Sequence Check(s)* Source pUA66 Contains promoterless gfp with rrnB T1 terminator, kanR resistance cassette, and pSC101 ori.
Gel Extraction:Article Title: An investigation of gene dosage reveals that increased sensitivity to D-cycloserine divergently impacts the transience of heteroresistance in Escherichia coli
Article Snippet: .. Product/component Manufacturer Difco LB Broth, Miller (Luria-Bertani) (Premix) Thermo Fisher BBL Cation-Adjust Mueller Hinton II Agar Premix Thermo Fisher Tryptone Fisher Bioreagents Yeast Extract Fisher Bioreagents Agar (80-100 mesh) Fisher Bioreagents D-glucose Fisher Chemical L-arabinose Alfa Aesar Glycerol Sigma Aldrich Chlorform Fisher Chemical Sodium Citrate Dihydrate (Na3C6H5O7*2H2O) Fisher Scientific Potassium Chloride (KCl) Fisher Sodium Chloride (NaCl) Fisher Bioreagents Calcium Chloride Dihydrate (CaCl2) Fisher Bioreagents Magnesium Sulfate Heptahydrate (MgSO4*7H2O) MP Biochemicals Sodium Phosphate Dibasic Heptahydrate (Na2HPO4*7H2O) MP Biochemicals Potassium dihyrogen Phosphate (KH2PO4) Alfa Aesar Ammonium Chloride (NH4Cl) MP biochemicals Gluose-6-phosphate (G6P) Sigma Nicotinamide (NIC) Sigma Aldrich Ciprofloxacin (CIP) Sigma Fosfomycin disodium salt (FOS) Sigma Aldrich Tobramycin sulfate (TOB) Sigma Aldrich D-cycloserine (DCS) Sigma Aldrich Anhydrotetracycline hydrochloride (aTET) Sigma Aldrich Kanamycin sulfate (KAN) Gibco Ampicillin (AMP) Acros Organics Lysozyme, chicken egg white Thermo Fisher Tris Hydrochloride (1 M, pH 8.0, DNase, RNase, and Protease Free) Fisher Bioreagents Ethylenediamine Tetraacetate Acid (EDTA) (for DNA work) (0.5 M, pH 8.0, RNase and Protease Free) Fisher Bioreagents Water (nuclease-free, 0.1 μm filtered, for molecular biology) Sigma Aldrich Gibson Assembly Master Mix New Englad Biolabs Phusion High Fidelity PCR Kit New Englad Biolabs Taq DNA Polymerase New Englad Biolabs EcoRI-HF Restriction Enzyme New Englad Biolabs 10x rCutSmart Buffer New Englad Biolabs Q5 Site-Directed Mutagenesis Kit New Englad Biolabs HiScibe T7 RNA Synthesis Kit New Englad Biolabs TaqMan Reverse Transcription Reagents Thermo Fisher Scientific Power SYBR Green qPCR Master Mix Applied Biosystems Qiaprep Spin Miniprep Kit Qiagen Qiaquick Gel Extraction Kit Qiagen Dneasy Blood & Tissue Kit Qiagen RNase-free DNase Kit Qiagen RNeasy Mini Kit Qiagen RNAprotect Bacteria Reagent Qiagen Plasmid Relevant description† PCR/Sequence Check(s)* Source pUA66 Contains promoterless gfp with rrnB T1 terminator, kanR resistance cassette, and pSC101 ori. .. Product/component Manufacturer Difco LB Broth, Miller (Luria-Bertani) (Premix) Thermo Fisher BBL Cation-Adjust Mueller Hinton II Agar Premix Thermo Fisher Tryptone Fisher Bioreagents Yeast Extract Fisher Bioreagents Agar (80-100 mesh) Fisher Bioreagents D-glucose Fisher Chemical L-arabinose Alfa Aesar Glycerol Sigma Aldrich Chlorform Fisher Chemical Sodium Citrate Dihydrate (Na3C6H5O7*2H2O) Fisher Scientific Potassium Chloride (KCl) Fisher Sodium Chloride (NaCl) Fisher Bioreagents Calcium Chloride Dihydrate (CaCl2) Fisher Bioreagents Magnesium Sulfate Heptahydrate (MgSO4*7H2O) MP Biochemicals Sodium Phosphate Dibasic Heptahydrate (Na2HPO4*7H2O) MP Biochemicals Potassium dihyrogen Phosphate (KH2PO4) Alfa Aesar Ammonium Chloride (NH4Cl) MP biochemicals Gluose-6-phosphate (G6P) Sigma Nicotinamide (NIC) Sigma Aldrich Ciprofloxacin (CIP) Sigma Fosfomycin disodium salt (FOS) Sigma Aldrich Tobramycin sulfate (TOB) Sigma Aldrich D-cycloserine (DCS) Sigma Aldrich Anhydrotetracycline hydrochloride (aTET) Sigma Aldrich Kanamycin sulfate (KAN) Gibco Ampicillin (AMP) Acros Organics Lysozyme, chicken egg white Thermo Fisher Tris Hydrochloride (1 M, pH 8.0, DNase, RNase, and Protease Free) Fisher Bioreagents Ethylenediamine Tetraacetate Acid (EDTA) (for DNA work) (0.5 M, pH 8.0, RNase and Protease Free) Fisher Bioreagents Water (nuclease-free, 0.1 μm filtered, for molecular biology) Sigma Aldrich Gibson Assembly Master Mix New Englad Biolabs Phusion High Fidelity PCR Kit New Englad Biolabs Taq DNA Polymerase New Englad Biolabs EcoRI-HF Restriction Enzyme New Englad Biolabs 10x rCutSmart Buffer New Englad Biolabs Q5 Site-Directed Mutagenesis Kit New Englad Biolabs HiScibe T7 RNA Synthesis Kit New Englad Biolabs TaqMan Reverse Transcription Reagents Thermo Fisher Scientific Power SYBR Green qPCR Master Mix Applied Biosystems Qiaprep Spin Miniprep Kit Qiagen Qiaquick Gel Extraction Kit Qiagen Dneasy Blood & Tissue Kit Qiagen RNase-free DNase Kit Qiagen RNeasy Mini Kit Qiagen RNAprotect Bacteria Reagent Qiagen Plasmid Relevant description† PCR/Sequence Check(s)* Source pUA66 Contains promoterless gfp with rrnB T1 terminator, kanR resistance cassette, and pSC101 ori.
Bacteria:Article Title: An investigation of gene dosage reveals that increased sensitivity to D-cycloserine divergently impacts the transience of heteroresistance in Escherichia coli
Article Snippet: .. Product/component Manufacturer Difco LB Broth, Miller (Luria-Bertani) (Premix) Thermo Fisher BBL Cation-Adjust Mueller Hinton II Agar Premix Thermo Fisher Tryptone Fisher Bioreagents Yeast Extract Fisher Bioreagents Agar (80-100 mesh) Fisher Bioreagents D-glucose Fisher Chemical L-arabinose Alfa Aesar Glycerol Sigma Aldrich Chlorform Fisher Chemical Sodium Citrate Dihydrate (Na3C6H5O7*2H2O) Fisher Scientific Potassium Chloride (KCl) Fisher Sodium Chloride (NaCl) Fisher Bioreagents Calcium Chloride Dihydrate (CaCl2) Fisher Bioreagents Magnesium Sulfate Heptahydrate (MgSO4*7H2O) MP Biochemicals Sodium Phosphate Dibasic Heptahydrate (Na2HPO4*7H2O) MP Biochemicals Potassium dihyrogen Phosphate (KH2PO4) Alfa Aesar Ammonium Chloride (NH4Cl) MP biochemicals Gluose-6-phosphate (G6P) Sigma Nicotinamide (NIC) Sigma Aldrich Ciprofloxacin (CIP) Sigma Fosfomycin disodium salt (FOS) Sigma Aldrich Tobramycin sulfate (TOB) Sigma Aldrich D-cycloserine (DCS) Sigma Aldrich Anhydrotetracycline hydrochloride (aTET) Sigma Aldrich Kanamycin sulfate (KAN) Gibco Ampicillin (AMP) Acros Organics Lysozyme, chicken egg white Thermo Fisher Tris Hydrochloride (1 M, pH 8.0, DNase, RNase, and Protease Free) Fisher Bioreagents Ethylenediamine Tetraacetate Acid (EDTA) (for DNA work) (0.5 M, pH 8.0, RNase and Protease Free) Fisher Bioreagents Water (nuclease-free, 0.1 μm filtered, for molecular biology) Sigma Aldrich Gibson Assembly Master Mix New Englad Biolabs Phusion High Fidelity PCR Kit New Englad Biolabs Taq DNA Polymerase New Englad Biolabs EcoRI-HF Restriction Enzyme New Englad Biolabs 10x rCutSmart Buffer New Englad Biolabs Q5 Site-Directed Mutagenesis Kit New Englad Biolabs HiScibe T7 RNA Synthesis Kit New Englad Biolabs TaqMan Reverse Transcription Reagents Thermo Fisher Scientific Power SYBR Green qPCR Master Mix Applied Biosystems Qiaprep Spin Miniprep Kit Qiagen Qiaquick Gel Extraction Kit Qiagen Dneasy Blood & Tissue Kit Qiagen RNase-free DNase Kit Qiagen RNeasy Mini Kit Qiagen RNAprotect Bacteria Reagent Qiagen Plasmid Relevant description† PCR/Sequence Check(s)* Source pUA66 Contains promoterless gfp with rrnB T1 terminator, kanR resistance cassette, and pSC101 ori. .. Product/component Manufacturer Difco LB Broth, Miller (Luria-Bertani) (Premix) Thermo Fisher BBL Cation-Adjust Mueller Hinton II Agar Premix Thermo Fisher Tryptone Fisher Bioreagents Yeast Extract Fisher Bioreagents Agar (80-100 mesh) Fisher Bioreagents D-glucose Fisher Chemical L-arabinose Alfa Aesar Glycerol Sigma Aldrich Chlorform Fisher Chemical Sodium Citrate Dihydrate (Na3C6H5O7*2H2O) Fisher Scientific Potassium Chloride (KCl) Fisher Sodium Chloride (NaCl) Fisher Bioreagents Calcium Chloride Dihydrate (CaCl2) Fisher Bioreagents Magnesium Sulfate Heptahydrate (MgSO4*7H2O) MP Biochemicals Sodium Phosphate Dibasic Heptahydrate (Na2HPO4*7H2O) MP Biochemicals Potassium dihyrogen Phosphate (KH2PO4) Alfa Aesar Ammonium Chloride (NH4Cl) MP biochemicals Gluose-6-phosphate (G6P) Sigma Nicotinamide (NIC) Sigma Aldrich Ciprofloxacin (CIP) Sigma Fosfomycin disodium salt (FOS) Sigma Aldrich Tobramycin sulfate (TOB) Sigma Aldrich D-cycloserine (DCS) Sigma Aldrich Anhydrotetracycline hydrochloride (aTET) Sigma Aldrich Kanamycin sulfate (KAN) Gibco Ampicillin (AMP) Acros Organics Lysozyme, chicken egg white Thermo Fisher Tris Hydrochloride (1 M, pH 8.0, DNase, RNase, and Protease Free) Fisher Bioreagents Ethylenediamine Tetraacetate Acid (EDTA) (for DNA work) (0.5 M, pH 8.0, RNase and Protease Free) Fisher Bioreagents Water (nuclease-free, 0.1 μm filtered, for molecular biology) Sigma Aldrich Gibson Assembly Master Mix New Englad Biolabs Phusion High Fidelity PCR Kit New Englad Biolabs Taq DNA Polymerase New Englad Biolabs EcoRI-HF Restriction Enzyme New Englad Biolabs 10x rCutSmart Buffer New Englad Biolabs Q5 Site-Directed Mutagenesis Kit New Englad Biolabs HiScibe T7 RNA Synthesis Kit New Englad Biolabs TaqMan Reverse Transcription Reagents Thermo Fisher Scientific Power SYBR Green qPCR Master Mix Applied Biosystems Qiaprep Spin Miniprep Kit Qiagen Qiaquick Gel Extraction Kit Qiagen Dneasy Blood & Tissue Kit Qiagen RNase-free DNase Kit Qiagen RNeasy Mini Kit Qiagen RNAprotect Bacteria Reagent Qiagen Plasmid Relevant description† PCR/Sequence Check(s)* Source pUA66 Contains promoterless gfp with rrnB T1 terminator, kanR resistance cassette, and pSC101 ori.
Plasmid Preparation:Article Title: An investigation of gene dosage reveals that increased sensitivity to D-cycloserine divergently impacts the transience of heteroresistance in Escherichia coli
Article Snippet: .. Product/component Manufacturer Difco LB Broth, Miller (Luria-Bertani) (Premix) Thermo Fisher BBL Cation-Adjust Mueller Hinton II Agar Premix Thermo Fisher Tryptone Fisher Bioreagents Yeast Extract Fisher Bioreagents Agar (80-100 mesh) Fisher Bioreagents D-glucose Fisher Chemical L-arabinose Alfa Aesar Glycerol Sigma Aldrich Chlorform Fisher Chemical Sodium Citrate Dihydrate (Na3C6H5O7*2H2O) Fisher Scientific Potassium Chloride (KCl) Fisher Sodium Chloride (NaCl) Fisher Bioreagents Calcium Chloride Dihydrate (CaCl2) Fisher Bioreagents Magnesium Sulfate Heptahydrate (MgSO4*7H2O) MP Biochemicals Sodium Phosphate Dibasic Heptahydrate (Na2HPO4*7H2O) MP Biochemicals Potassium dihyrogen Phosphate (KH2PO4) Alfa Aesar Ammonium Chloride (NH4Cl) MP biochemicals Gluose-6-phosphate (G6P) Sigma Nicotinamide (NIC) Sigma Aldrich Ciprofloxacin (CIP) Sigma Fosfomycin disodium salt (FOS) Sigma Aldrich Tobramycin sulfate (TOB) Sigma Aldrich D-cycloserine (DCS) Sigma Aldrich Anhydrotetracycline hydrochloride (aTET) Sigma Aldrich Kanamycin sulfate (KAN) Gibco Ampicillin (AMP) Acros Organics Lysozyme, chicken egg white Thermo Fisher Tris Hydrochloride (1 M, pH 8.0, DNase, RNase, and Protease Free) Fisher Bioreagents Ethylenediamine Tetraacetate Acid (EDTA) (for DNA work) (0.5 M, pH 8.0, RNase and Protease Free) Fisher Bioreagents Water (nuclease-free, 0.1 μm filtered, for molecular biology) Sigma Aldrich Gibson Assembly Master Mix New Englad Biolabs Phusion High Fidelity PCR Kit New Englad Biolabs Taq DNA Polymerase New Englad Biolabs EcoRI-HF Restriction Enzyme New Englad Biolabs 10x rCutSmart Buffer New Englad Biolabs Q5 Site-Directed Mutagenesis Kit New Englad Biolabs HiScibe T7 RNA Synthesis Kit New Englad Biolabs TaqMan Reverse Transcription Reagents Thermo Fisher Scientific Power SYBR Green qPCR Master Mix Applied Biosystems Qiaprep Spin Miniprep Kit Qiagen Qiaquick Gel Extraction Kit Qiagen Dneasy Blood & Tissue Kit Qiagen RNase-free DNase Kit Qiagen RNeasy Mini Kit Qiagen RNAprotect Bacteria Reagent Qiagen Plasmid Relevant description† PCR/Sequence Check(s)* Source pUA66 Contains promoterless gfp with rrnB T1 terminator, kanR resistance cassette, and pSC101 ori. .. Product/component Manufacturer Difco LB Broth, Miller (Luria-Bertani) (Premix) Thermo Fisher BBL Cation-Adjust Mueller Hinton II Agar Premix Thermo Fisher Tryptone Fisher Bioreagents Yeast Extract Fisher Bioreagents Agar (80-100 mesh) Fisher Bioreagents D-glucose Fisher Chemical L-arabinose Alfa Aesar Glycerol Sigma Aldrich Chlorform Fisher Chemical Sodium Citrate Dihydrate (Na3C6H5O7*2H2O) Fisher Scientific Potassium Chloride (KCl) Fisher Sodium Chloride (NaCl) Fisher Bioreagents Calcium Chloride Dihydrate (CaCl2) Fisher Bioreagents Magnesium Sulfate Heptahydrate (MgSO4*7H2O) MP Biochemicals Sodium Phosphate Dibasic Heptahydrate (Na2HPO4*7H2O) MP Biochemicals Potassium dihyrogen Phosphate (KH2PO4) Alfa Aesar Ammonium Chloride (NH4Cl) MP biochemicals Gluose-6-phosphate (G6P) Sigma Nicotinamide (NIC) Sigma Aldrich Ciprofloxacin (CIP) Sigma Fosfomycin disodium salt (FOS) Sigma Aldrich Tobramycin sulfate (TOB) Sigma Aldrich D-cycloserine (DCS) Sigma Aldrich Anhydrotetracycline hydrochloride (aTET) Sigma Aldrich Kanamycin sulfate (KAN) Gibco Ampicillin (AMP) Acros Organics Lysozyme, chicken egg white Thermo Fisher Tris Hydrochloride (1 M, pH 8.0, DNase, RNase, and Protease Free) Fisher Bioreagents Ethylenediamine Tetraacetate Acid (EDTA) (for DNA work) (0.5 M, pH 8.0, RNase and Protease Free) Fisher Bioreagents Water (nuclease-free, 0.1 μm filtered, for molecular biology) Sigma Aldrich Gibson Assembly Master Mix New Englad Biolabs Phusion High Fidelity PCR Kit New Englad Biolabs Taq DNA Polymerase New Englad Biolabs EcoRI-HF Restriction Enzyme New Englad Biolabs 10x rCutSmart Buffer New Englad Biolabs Q5 Site-Directed Mutagenesis Kit New Englad Biolabs HiScibe T7 RNA Synthesis Kit New Englad Biolabs TaqMan Reverse Transcription Reagents Thermo Fisher Scientific Power SYBR Green qPCR Master Mix Applied Biosystems Qiaprep Spin Miniprep Kit Qiagen Qiaquick Gel Extraction Kit Qiagen Dneasy Blood & Tissue Kit Qiagen RNase-free DNase Kit Qiagen RNeasy Mini Kit Qiagen RNAprotect Bacteria Reagent Qiagen Plasmid Relevant description† PCR/Sequence Check(s)* Source pUA66 Contains promoterless gfp with rrnB T1 terminator, kanR resistance cassette, and pSC101 ori.
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